quantitative bisulfite pyrosequencing method  (Pyrosequencing Inc)

Journal: Development & Reproduction

Article Title: Overexpression of Hypermethylated Homeobox A11 (HOXA11) Inhibits Tumor Cell Growth and Induces Apoptosis in Cervical Cancer

doi: 10.12717/DR.2024.28.2.37

Figure Lengend Snippet: Quantitative methylation analysis of HOXA11 promoter in cervical scrapes (n=387) representing normal, various cervical dysplasia and biopsy-confirmed invasive cervical cancer. Average methylation percentages on the HOXA11 promoter are represented by box and whisker plots.

Article Snippet: To assess methylation status of HOXA11 gene, quantitative bisulfite-pyrosequencing ( ) was performed.

Techniques: Methylation, Whisker Assay

Journal: Development & Reproduction

Article Title: Overexpression of Hypermethylated Homeobox A11 (HOXA11) Inhibits Tumor Cell Growth and Induces Apoptosis in Cervical Cancer

doi: 10.12717/DR.2024.28.2.37

Figure Lengend Snippet: Cervical cancer cells were seeded on 24-well culture plates and attached for 24 hours. HeLa and HT3 cancer cells were transiently transfected with expression vectors encoding HOXA11 and the number of survival cells was counted at 1 day intervals for 3 days. Results are means±SD from three samples each. The cells were observed at 40× magnification.

Article Snippet: To assess methylation status of HOXA11 gene, quantitative bisulfite-pyrosequencing ( ) was performed.

Techniques: Transfection, Expressing

Journal: Development & Reproduction

Article Title: Overexpression of Hypermethylated Homeobox A11 (HOXA11) Inhibits Tumor Cell Growth and Induces Apoptosis in Cervical Cancer

doi: 10.12717/DR.2024.28.2.37

Figure Lengend Snippet: HeLa cells were transiently transfected with control pcDNA3.1 and pcDNA3.1-HOXA11. (A) Mitochondrial and cytosolic fractions were collected at 48 hrs after transfection and quantified cytochrome C concentration by western hybridization. (B) HeLa cells were transiently transfected with pcDNA3.1-HOXA11, then harvested at 24 hrs and 48 hrs after transfection and analyzed by western blot. The expression levels of HOXA11, Bax, cleaved PARP, c-Myc, VEGF, and GAPDH were measured.

Article Snippet: To assess methylation status of HOXA11 gene, quantitative bisulfite-pyrosequencing ( ) was performed.

Techniques: Transfection, Control, Concentration Assay, Western Blot, Hybridization, Expressing

Journal: Journal of Alzheimer's Disease Reports

Article Title: Utility of DNA Methylation as a Biomarker in Aging and Alzheimer’s Disease

doi: 10.3233/ADR-220109

Figure Lengend Snippet: Summary of DNA methylation studies in AD

Article Snippet: AD , Peripheral blood , 83 (43 case, 38 control) , 55-87 , Quantitative bisulfite-PCR pyrosequencing , Targeted repetitive elements , LINE-1 methylation increased in AD compared to controls , [ ] .

Techniques: DNA Methylation Assay, Southern Blot, Control, Immunohistochemistry, Methylation, Mass Spectrometry, Methylation Sequencing, Biomarker Discovery, Fluorescence, High Content Screening, Enzyme-linked Immunosorbent Assay, Cloning, Sequencing, Gene Expression, Luciferase